Composite

Part:BBa_K3081044:Design

Designed by: Le Zhang   Group: iGEM19_Peking   (2019-10-16)


pBAD-dCas9-ssrA-J23119-NT1-J23119-mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 2321
    Illegal NheI site found at 5495
    Illegal NheI site found at 5518
    Illegal NheI site found at 5626
    Illegal NheI site found at 5649
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 4600
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 6209
    Illegal AgeI site found at 6321
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

No


Source

E.coli

References